Creates a new RestrictionEnzyme of the unusual type which cuts both upstream and downstream of its recognition site.

Namespace:  BioSharp.Core.Bio.Molbio
Assembly:  BioSharp.Core (in BioSharp.Core.dll) Version: 0.1.3191.26120 (0.1.0.0)

Syntax

C#
public RestrictionEnzyme(
	string name,
	ISymbolList site,
	int usForward,
	int usReverse,
	int dsForward,
	int dsReverse
)

Parameters

name
Type: System..::.String
a String such as Bsp24I.
site
Type: BioSharp.Core.Bio.Symbol..::.ISymbolList
a SymbolList recognition site.
usForward
Type: System..::.Int32
an int index in the forward strand (the strand conventionally written 5'-3') upstream of the recognition site at which the cut occurs. The cut occurs between this base and the following one.
usReverse
Type: System..::.Int32
an int index in the reverse strand (the strand conventionally written 3'-5') upstream of the recognition site at which the cut occurs. The cut occurs between this base and the following one.
dsForward
Type: System..::.Int32
an int index in the forward strand (the strand conventionally written 5'-3') downstream of the recognition site at which the cut occurs. The cut occurs between this base and the following one.
dsReverse
Type: System..::.Int32
an int index in the reverse strand (the strand conventionally written 3'-5') downstream of the recognition site at which the cut occurs. The cut occurs between this base and the following one.

Remarks

The cut position indices are always in the same coordinate space as the recognition site.

See Also