Creates a new RestrictionEnzyme which cuts within or downstream of the recognition site.

Namespace:  BioSharp.Core.Bio.Molbio
Assembly:  BioSharp.Core (in BioSharp.Core.dll) Version: 0.1.3191.26120 (0.1.0.0)

Syntax

C#
public RestrictionEnzyme(
	string name,
	ISymbolList site,
	int dsForward,
	int dsReverse
)

Parameters

name
Type: System..::.String
a String such as EcoRI.
site
Type: BioSharp.Core.Bio.Symbol..::.ISymbolList
an ISymbolList recognition site.
dsForward
Type: System..::.Int32
an int index in the forward strand (the strand conventionally written 5'-3') of the recognition site at which the cut occurs. The cut occurs between this base and the following one.
dsReverse
Type: System..::.Int32
an int index in the reverse strand (the strand conventionally written 3'-5') of the recognition site at which the cut occurs. The cut occurs between this base and the following one.

Remarks

The cut position indices are always in the same coordinate space as the recognition site. RestrictionEnzymes are immutable.

See Also